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Custom Polyclonal Antibody Services (Mouse)

         Custom Polyclonal Antibody Services (Mouse)

                                 

Starting From

To

Anti-sera

Protein A Purified IgGs

 

Quantity

QC Data

Code

AS

PA

Peptide antigens from customers

~ 5 mg

>85% Pure by HPLC Correct MW by MS

MPE

Inquiry

(4-12 weeks)

 

~10mg

 

Inquiry

(4-12 weeks)

Protein antigens from customers

~ 5mg

> 90% Pure and correct MW by SDS-PAGE

MPR

Inquiry

(4-12 weeks)

 

~10mg

 

Inquiry

(4-12 weeks)

Peptide sequences (peptide synthesis at EnoGene)

~15mg

>85% Pure by HPLC Correct MW by MS

MPS

Inquiry (11-14 weeks)

Inquiry

(11-14 weeks)

Protein sequences (Peptide design and synthesis at EnoGene)

MPD

 

1. MPE-AS or MPR-AS: Basic Polyclonal Antibody Package (Mouse)

Includes

  • Conjugation to KLH (optional)
  • Five Balb/c mice, pre-immune bleed, four immunizations/mouse
  • ELISA test
  • Antigens provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and the antigen solution must not contain organic solvents.

Delivery

  • 10-20 µl of pre-immune serum
  • ELISA results
  • 1-2.5 ml of antisera from all mice

Time frame

  • Conjugation: 1 week
  • Immunization and ELISA: 11 weeks
  • Shipping: 1 week
  • Total 12-13 weeks

2. MPE-PA or MPR-PA: Basic Protein A-Purified Polyclonal Antibody Package (Mouse)

Includes

  • Conjugation to KLH (optional)
  • 5 Balb/c mice, pre-immune bleed, 4 immunizations/mouse
  • Final bleed
  • Protein A purification
  • ELISA test
  • Antigen provided by the customer: 2.5-3 mg of peptide or 1.5 mg protein (>85% purity, 90% preferred) is required. The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution.

Delivery

  • 10-20 µl of pre-immune serum
  • ELISA results
  • 2.5-12.5 mg protein A-purified antibodies
  • Remained antigen

Time frame

  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Protein A Purification and ELISA: 1-2 week
  • Shipping: 1 week
  • Total 13-14 weeks

3. MPS-AS or MPD-AS: Complete Peptide Polyclonal Antibody Package (Mouse)

Includes

  • Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Five Balb/c mice, pre-immune bleed, four immunizations/mouse
  • ELISA test

Delivery

  • 10-20 µl of pre-immune serum
  • ELISA results
  • 1-2.5 ml of antisera from all mice
  • Remained unconjugated peptide, MS and HPLC report

Time frame

  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization and ELISA: 11 weeks
  • Shipping: 1 week
  • Total 14-16 weeks

4. MPS-PA or MPD-PA: Complete Protein A-Purified Peptide Polyclonal Antibody Package (Mouse)

Includes

  • Peptide synthesis (≤15 a.a, >85% purity, 15 mg)
  • A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH.
  • Five Balb/C mice, pre-immune, four immunizations/mouse
  • Antibody purification by protein A column
  • ELISA test

Delivery

  • 10-20 µl of pre-immune serum
  • ELISA results
  • 2.5-12.5 mg purified antibody from all antisera
  • Remained unconjugated peptide, MS and HPLC report

Time frame

  • Peptide Synthesis: 2-3 weeks
  • Conjugation: 1 week
  • Immunization: 10 weeks
  • Purification and ELISA: 1-2week
  • Shipping: 1 week
  • Total 15-17 weeks
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