Form of Antibody:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7。4, 150mM NaCl, 0。02% sodium azide and 50% glycerol。
Storage/Stability:Store at -20℃/1 year
Immunogen:The antiserum was produced against synthesized phosphopeptide derived from human ERK1/2 around the phosphorylation site of Thr202/Tyr204 (F-L-TP-E -YP-V-A)。
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide。 The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site。
Specificity/Sensitivity:ERK1/2 (phospho-Thr202/Tyr204) antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr202/Tyr204.
Reactivity:Human, Mouse, Rat
Applications:WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:4000
References:Marais, R. et al. (1993) Cell 73, 381-93.
Research Area:Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells