Form of Antibody:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage/Stability:Store at -20℃/1 year
Immunogen:The antiserum was produced against synthesized phosphopeptide derived from human GSK3α around the phosphorylation site of serine 21 (T-S-SP-F-A).
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide。 The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site。
Specificity/Sensitivity:GSK3α (phospho-Ser21) antibody detects endogenous levels of GSK3α only when phosphorylated at serine 21.
Reactivity:Human, Mouse, Rat
幸运飞艇官网Applications:WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100-200
References:Barry FA, et al. (2003) FEBS Lett. 553(1-2): 173-178.
Research Area:Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells