Form of Antibody:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7。4, 150mM NaCl, 0。02% sodium azide and 50% glycerol。
Storage/Stability:Store at -20℃/1 year
Immunogen:The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 383 (T-L-SP-P-I)。
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Specificity/Sensitivity:Elk-1 (phospho-Ser383) antibody detects endogenous levels of Elk-1 only when phosphorylated at serine 383.
Reactivity:Human, Mouse, Rat
Applications:WB: 1:500~1:1000 IHC: 1:50~1:100
References:Xing J, et al. (1996) Science. 273(5277): 959-963.
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