Form of Antibody:Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7。4, 150mM NaCl, 0。02% sodium azide and 50% glycerol。
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Immunogen:The antiserum was produced against synthesized phosphopeptide derived from human c-Jun around the phosphorylation site of serine 63 (L-T-SP-P-D)。
Purification:The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Specificity/Sensitivity:c-Jun (phospho-Ser63) antibody detects endogenous levels of c-Jun only when phosphorylated at serine 63。
Applications:WB: 1:500~1:1000 IHC: 1:50~1:100
References:Wei W, et al. (2005). Cancer Cell. 8(1): 25-33.
Research Area:Autophagy antibody Cancer Cardiovascular Cell Biology Epigenetics & Nuclear Signaling Developmental Biologys Immunology Drug Discovery Products Metabolism Neuroscience Signal Transduction Stem Cells